Assessment of a Simple Competitive Protein-Binding Technique for Plasma Cortisol Assay
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چکیده
منابع مشابه
Rapid, specific assay for plasma cortisol by competitive protein binding.
We describe a modified competitive protein-binding method for assay of plasma cortisol. Plasma samples are deproteinized by dilution with an ethanol/phosphate buffer, followed by heating at 100 degrees C for 2 min. Horse serum is used as the source of transcortin. Free radioactivity is separated from the protein-bound component by partition into liquid scintillation counting within 60 min. The ...
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A simple radioligand assay has been developed for measuring cortisol in plasma and urine. The antiserum was produced in rabbits by using cortisoi-21hemisuccinyl-bovine serum albumin as the antigen. The standard curve was linear from 0-1000 pg. A small aliquot of ethanol-precipitated plasma or urine is directly assayed. Interassay variability of 56 separate assays of a normal plasma pool during ...
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A method is described for quantitation of testosterone in plasma from females and males. The principal operations of extraction, chromatographic fractionation, and competitive protein-binding assay can be completed for eight duplicate samples in a single day. Experimental data used in the development of the test and the rationale for the specific operations and conditions are presented. The spe...
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1. A competitive protein-binding assay for plasma 25-hydroxycholecalciferol was developed, using Sephadex LH-20 columns for the isolation of the sterol and a partially purified protein from rat serum as the binding protein. 2. The mean plasma 25-hydroxycholecalciferol in eighteen male and female normal volunteers was 38 5 SD 14 pmol/ml (1 5.2 & 5.6 nglml). 3. Levels of 25-hydroxycholecalciferol...
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We describe an improved competitive protein-binding radioassay for total plasma cortisol, which permits rapid analysis of many samples. Plasma, 0.1 mL, is directly applied to a Whatman 3MM filter-paper strip, which is extracted once with dichloromethane. The extract is evaporated and the residue is assayed directly by the micro-method of Murphy (J. Clin. Endocrinol. Metab. 27: 973, 1967). Compa...
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ژورنال
عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
سال: 1975
ISSN: 0004-5632,1758-1001
DOI: 10.1177/000456327501200120